Sample data in (two factor with blocking factor and 3 reference genes)

A sample qPCR data set with blocking factor and 3 reference genes. Each line belongs to a separate individual (non-repeated measure experiment).

Usage

data_2factorBlock3ref

Format

A data frame with 18 observations and 8 variables:

Type

First experimental factor

Concentration

Second experimental factor

block

blocking factor

Rep

Biological replicates

PO

Mean amplification efficiency of PO gene

Ct_PO

Ct values of PO gene. Each is the mean of technical replicates

NLM

Mean amplification efficiency of NLM gene

Ct_NLM

Ct values of NLM gene. Each is the mean of technical replicates

ref1

Mean amplification efficiency of ref1 gene

Ct_ref1

Ct values of ref1 gene. Each is the mean of technical replicates

ref2

Mean amplification efficiency of ref2 gene

Ct_ref2

Ct values of ref2 gene. Each is the mean of technical replicates

ref3

Mean amplification efficiency of ref3 gene

Ct_ref3

Ct values of GAPDH gene. Each is the mean of technical replicates

Source

Not applicable